產品名稱：Human IFN-gamma
產品型號：SEKH-0046
產品報價：
產品特點：Human IFN-gamma索萊寶ELISA試劑盒的優(yōu)勢：
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Human IFN-gamma
| 中文名稱 | 人干擾素γ檢測試劑盒 |
|---|---|
| 英文名稱 | Human IFN-γ ELISA KIT |
| Reactivity | Human |
| Sample type | Serum, plasma, Cell culture supernatant |
| Sensitivity | 4 pg/ml |
| Detection Target | IFN-γ |
| Applications | Sandwich ELISA |
Human IFN-gamma
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm
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